Neurobiology of Disease Spinal Dorsal Horn Calcium Channel 2 -1 Subunit Upregulation Contributes to Peripheral Nerve Injury- Induced Tactile Allodynia

Peripheral nerve injury induces upregulation of the calcium channel 2 -1 structural subunit in dorsal root ganglia (DRG) and dorsal spinal cord of spinal nerve-ligated rats with neuropathic pain, suggesting a role of the calcium channel 2 -1 subunit in central sensitization. To investigate whether spinal dorsal horn 2 -1 subunit upregulation derives from increased DRG 2 -1 subunit and plays a causal role in neuropathic pain development, we examined spinal dorsal horn 2 -1 subunit expression with or without dorsal rhizotomy in spinal nerve-ligated rats and its correlation with tactile allodynia, a neuropathic pain state defined as reduced thresholds to nonnoxious tactile stimulation. We also examined the effects of intrathecal 2 -1 antisense oligonucleotides on 2 -1 subunit expression and neuropathic allodynia in the nerve-ligated rats. Our data indicated that spinal nerve injury resulted in time-dependent 2 -1 subunit upregulation in the spinal dorsal horn that correlated temporally with neuropathic allodynia development and maintenance. Dorsal rhizotomy diminished basal level expression and blocked injury-induced expression of the spinal dorsal horn 2 -1 subunit and reversed injury-induced tactile allodynia. In addition, intrathecal 2 -1 antisense oligonucleotides blocked injury-induced dorsal horn 2 -1 subunit upregulation and diminished tactile allodynia. These findings indicate that 2 -1 subunit basal expression occurs presynaptically and postsynaptically in spinal dorsal horn. Nerve injury induces mainly presynaptic 2 -1 subunit expression that derives from increased 2 -1 subunit in injured DRG neurons. Thus, changes in presynaptic 2 -1 subunit expression contribute to injury-induced spinal neuroplasticity and central sensitization that underlies neuropathic pain development and maintenance.